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Targeting transcription factor binding sites of the interleukin 7 receptor gene using CRISPR/CAS9 genome editing

Çetin, Ronay (2017) Targeting transcription factor binding sites of the interleukin 7 receptor gene using CRISPR/CAS9 genome editing. [Thesis]

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Official URL: http://risc01.sabanciuniv.edu/record=b1655701 (Table of Contents)

Abstract

The interleukin 7 cytokine receptor protein (IL-7R) encoded by the il-7r gene is expressed in immune system cells and it allows these cells to be signaled by the IL-7 survival factor. Mutations in the IL-7R pathway are associated with primary immunodeficiency diseases. Polymorphisms in the IL-7R gene are directly linked to T-cell acute lymphoblastic leukemia (T-ALL) and multiple sclerosis (MS). These findings point to the importance of this receptor in health. We have assessed the transcription mechanism of the IL-7R gene in T lymphocytes. We identified the functional significance of transcription factors that bind to IL-7R gene control elements. To this end, we generated mutations in the IL- 7R gene by using CRISPR/Cas9 mediated genome editing. We targeted NF-kB binding site in the enhancer and RORgT and Gfi1 binding sites in intron 2 of the IL-7R gene. We identified the significance of these mutations on IL-7R expression. We performed chromatin immunoprecipitation (ChIP) experiments to reveal the binding abilities of transcription factors to the IL-7R gene targeted with CRISPR/Cas9. In this study, we identified important transcription factors that control the survival of lymphocytes of the immune system.

Item Type:Thesis
Additional Information:Yükseköğretim Kurulu Tez Merkezi Tez No: 478667.
Uncontrolled Keywords:T lymphocyte. -- Interleukin-7 receptor. -- CRISPR/Cas9. -- Genome engineering. -- Regulation of transcription. -- T lenfosit, İnterlökin-7 almacı. -- CRISPR/Cas9. -- Genom mühendisliği. -- Transkripsiyon düzenlenmesi.
Subjects:T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
ID Code:34692
Deposited By:IC-Cataloging
Deposited On:07 May 2018 14:27
Last Modified:07 May 2018 14:27

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