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Enhancing antioxidant cellular defense by using quercetin loaded TiO2 nanoparticles in Swiss 3T3 albino mouse fibroblast cells

Birinci, Yelda and Kolkar Mohammed, Javed Hussain Niazi and Başağa, Hüveyda (2017) Enhancing antioxidant cellular defense by using quercetin loaded TiO2 nanoparticles in Swiss 3T3 albino mouse fibroblast cells. Journal of Nanomedicine and Nanotechnology . ISSN 2157-7439

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Official URL: http://dx.doi.org/10.4172/2157-7439.S8-001

Abstract

Living organisms are continuously exposed to a wide variety of oxidative radicals. Therefore, there is a constant demand for exogenous antioxidant supply. In this regard, one of these exogenous antioxidants with well established potency, quercetin has been utilized in various formulations. Yet due to its poor water solubility, its extensive applications are limited so far. In this study, TiO2 nanoparticles (TiO2-NPs) were employed to improve the cellular penetration of quercetin and to maximize its antioxidant effects on swiss 3T3 albino mouse fibroblast cells. Surfaces of TiO2-NPs were modified with Polyethylene glycol (PEG) that enabled better dispersion and enhanced biocompatibility. Toxicity of quercetin and quercetin loaded TiO2 NPs (QL-TiO2-NPs) were evaluated in terms of cell morphology and measured with cell viability assay. For an in-depth cell viability analysis, key markers of apoptosis were investigated by immunoblotting analysis. As an indicator of apoptotic cell death, cleavage of caspase 3 (Cas 3) and poly (ADP-ribose) polymerase proteins (Parp) were detected in quercetin treated cells. Antioxidant capacity of quercetin in the form of QL-TiO2-NPs was measured in the cells in which generation of reactive oxygen species (ROS) and superoxide was induced by pyocyanin. Quantitative ROS measurements were confirmed with confocal microscopy. Further mechanistic insight on upregulation of NF-E2 related factor 2 (NRF2) pathway via QL-TiO2-NPs was also provided in an effort to validate its antioxidant defense. Target enzymes of NRF2, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxydoreductase1 (NQO1) and superoxide dismutase1 (SOD1) expressions were increased in the model proposed. QL-TiO2-NPs allowed high bioavaliability of quercetin concentration and stability in the cell with maximum antioxidant capacity against formation of ROS without any cytotoxicity. Overall, this paper sheds light on how the efficiency of quercetin in a nanosystem can serve as a safe therapeutic candidate for breaking the vicious ROS cycle in the cell.

Item Type:Article
Uncontrolled Keywords:Quercetin; TiO2 nanoparticles; ROS; Superoxide; NRF2; Quercetin topical application
Subjects:R Medicine > R Medicine (General)
R Medicine > RM Therapeutics. Pharmacology
ID Code:34200
Deposited By:Yelda Birinci
Deposited On:06 Feb 2018 15:42
Last Modified:06 Feb 2018 15:42

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