title   
  

Isoniazid killing of mycobacterium smegmatis NADH pyrophosphatase mutant at single-cell level using microfluidics and time-lapse microscopy

Elitaş, Meltem (2017) Isoniazid killing of mycobacterium smegmatis NADH pyrophosphatase mutant at single-cell level using microfluidics and time-lapse microscopy. Scientific Reports, 7 . ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/s41598-017-11503-7

Abstract

We introduce single-cell analysis for isoniazid-treated Mycobacterium smegmatis mutant, msm1946-NADH pyrophosphatase, using microfluidics and automated time-lapse microscopy. Mycobacterial NADH pyrophosphatase isoforms play an important role for the mechanism of isoniazid and ethionamide activation. Our single-cell analysis revealed important insights on isoniazid killing mechanism that was masked by traditional killing assays, raised significant questions related to viable but non-culturable subpopulation of cells, and existing methods that defines minimum inhibitory concentration of drugs. The major goal of this study was quantitatively analyze bacterial cell parameters to obtain high-resolution data for the time evolution of antibiotic killing at the single-cell level. The presented tools and methods could be applied to the closely related organisms to provide more detailed information for the design and employment of antibiotic treatments.

Item Type:Article
Subjects:Q Science > Q Science (General)
R Medicine > R Medicine (General) > R855-855.5 Medical technology
ID Code:33838
Deposited By:Meltem Elitaş
Deposited On:09 Sep 2017 20:07
Last Modified:09 Sep 2017 20:07

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