Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes
David, Iulia Gabriela and Buleandra, Mihaela and Popa, Dana Elena and Bizgan, Ana-Maria Cristina and Moldovan, Zenovia and Badea, Irinel-Adriana and Iorgulescu, Emilia Elena and Tekiner, Tuğçe Ayça and Başağa, Hüveyda (2016) Voltammetric determination of polyphenolic content as rosmarinic acid equivalent in tea samples using pencil graphite electrodes. Journal of Food Science and Technology, 53 (6). pp. 2589-2596. ISSN 0022-1155 (Print) 0975-8402 (Online)
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Official URL: http://dx.doi.org/10.1007/s13197-016-2223-y
The quasi-reversible, diffusion controlled behavior of rosmarinic acid (RA) on a disposable pencil graphite electrode (PGE) was established by cyclic voltammetry. Using the anodic oxidation peak presented by RA on the PGE a differential pulse voltammetric (DPV) method was developed for the quantitative determination of RA. The linear range was 10(-8) - 10(-5) M RA and the detection and quantification limits were 7.93 x 10(-9) M and 2.64 x 10(-8) M RA, respectively. The applicability of the developed method was tested by recovery studies and by the assessment of the total polyphenolic contents (TPCDPV) of green, white and black Turkish teas, which were found to be 40.74, 30.04 and 23.97 mg rosmarinic acid equivalent/g dry tea, respectively. These results were in good agreement with those obtained by the Folin-Ciocalteu method. The developed method is a sensitive and cheap tool for the rapid and precise evaluation of TPCDPV of tea samples.
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