title   
  

Label-free capacitance based aptasensor platform for the detection of HER2/ErbB2 cancer biomarker in serum

Qureshi, Anjum and Gürbüz, Yaşar and Kolkar Mohammed, Javed Hussain Niazi (2015) Label-free capacitance based aptasensor platform for the detection of HER2/ErbB2 cancer biomarker in serum. Sensors and Actuators B: Chemical, 220 . pp. 1145-1151. ISSN 0925-4005

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Official URL: http://dx.doi.org/10.1016/j.snb.2015.06.094

Abstract

In this study, a label-free capacitive aptasensor was developed based on capturing of human epidermal growth factor receptor 2 (HER2) protein by anti-HER2 ssDNA aptamers functionalized on interdigitated microelectrodes of capacitor as bio-recognition elements. The aptasensor response was measured by non-Faradaic Impedance Spectroscopy (nFIS) method. Capacitance signal specific to target HER2 protein is based on changes occurred due to charge distribution upon interaction of aptamer-protein molecules against to the applied AC frequency (50-350 MHz). HER2 protein in spiked human serum was successfully detected through concentration dependent changes in impedance/capacitance values as a result of the formation of aptamer-HER2 protein complex (0.2-2 ng mL(-1) of HER2) on capacitor microelectrodes. The label-free capacitive aptasensing is a versatile and promising approach for early detection of cancer biomarkers in dilute human serum and the method potentially be extended to a wide variety of diseases biomarkers.

Item Type:Article
Uncontrolled Keywords:HER2; Aptamer; Cancer biomarker; Biosensor; Cancer diagnosis
Subjects:R Medicine > R Medicine (General)
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Q Science > QD Chemistry
ID Code:27334
Deposited By:Javed Hussain Niazi Kolkar Mohammed
Deposited On:02 Dec 2015 15:34
Last Modified:02 Dec 2015 15:34

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