Assessment of a direct hybridization microarray strategy for comprehensive monitoring of genetically modified organisms (GMOs)
Türkeç, Aydın and Lucas, Stuart J. and Karacanlı, Burçin and Baykut, Aykut and Yüksel, Hakkı (2016) Assessment of a direct hybridization microarray strategy for comprehensive monitoring of genetically modified organisms (GMOs). Food Chemistry, 194 . pp. 399-409. ISSN 0308-8146
This is the latest version of this item.
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.foodchem.2015.08.030
Detection of GMO material in crop and food samples is the primary step in GMO monitoring and regulation, with the increasing number of GM events in the world market requiring detection solutions with high multiplexing capacity. In this study, we test the suitability of a high-density oligonucleotide microarray platform for direct, quantitative detection of GMOs found in the Turkish feed market. We tested 1830 different 60 nt probes designed to cover the GM cassettes from 12 different GM cultivars (3 soya, 9 maize), as well as plant species-specific and contamination controls, and developed a data analysis method aiming to provide maximum throughput and sensitivity. The system was able specifically to identify each cultivar, and in 10/12 cases was sensitive enough to detect GMO DNA at concentrations of ≤ 1%. These GMOs could also be quantified using the microarray, as their fluorescence signals increased linearly with GMO concentration.
Available Versions of this Item
Repository Staff Only: item control page