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Assessment of a direct hybridization microarray strategy for comprehensive monitoring of genetically modified organisms (GMOs)

Türkeç, Aydın and Lucas, Stuart J. and Karacanlı, Burçin and Baykut, Aykut and Yüksel, Hakkı (2016) Assessment of a direct hybridization microarray strategy for comprehensive monitoring of genetically modified organisms (GMOs). Food Chemistry, 194 . pp. 399-409. ISSN 0308-8146

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Official URL: http://dx.doi.org/10.1016/j.foodchem.2015.08.030

Abstract

Detection of GMO material in crop and food samples is the primary step in GMO monitoring and regulation, with the increasing number of GM events in the world market requiring detection solutions with high multiplexing capacity. In this study, we test the suitability of a high-density oligonucleotide microarray platform for direct, quantitative detection of GMOs found in the Turkish feed market. We tested 1830 different 60 nt probes designed to cover the GM cassettes from 12 different GM cultivars (3 soya, 9 maize), as well as plant species-specific and contamination controls, and developed a data analysis method aiming to provide maximum throughput and sensitivity. The system was able specifically to identify each cultivar, and in 10/12 cases was sensitive enough to detect GMO DNA at concentrations of ≤ 1%. These GMOs could also be quantified using the microarray, as their fluorescence signals increased linearly with GMO concentration.

Item Type:Article
Uncontrolled Keywords:Genetically modified organisms (GMOs); GMO detection; food and feed safety; microarray; comparative genomic hybridization (CGH); quantification
Subjects:Q Science > QH Natural history > QH506 Molecular biology
ID Code:27322
Deposited By:Stuart J. Lucas
Deposited On:29 Nov 2015 21:26
Last Modified:29 Nov 2015 21:26

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