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Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides

Akpınar, Bala Anı and Yüce, Meral and Lucas, Stuart J. and Vrana, Jan and Buresova, Veronika and Dolezel, Jaroslav and Budak, Hikmet (2015) Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides. Scientific Reports, 5 . ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/srep10763

Abstract

Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome 5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances, revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the increasing demand to feed the world.

Item Type:Article
Additional Information:Article Number: 10763
Subjects:Q Science > QH Natural history > QH506 Molecular biology
S Agriculture > SB Plant culture > SB183-317 Field crops
ID Code:27321
Deposited By:Stuart J. Lucas
Deposited On:20 Nov 2015 11:35
Last Modified:15 Dec 2015 16:07

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