The comparison of plant regeneration between Jerusalem artichoke and purple potato cultured on MS media with different concentrations and combinations of plant growth regulators

Abstract

A deeper understanding of the influence of culture media and different germplasm is crucial to propagate the plants in vitro conditions. To focus this, two tuberous plant species; Jerusalem artichoke (Helianthus tuberosus) and Purple potato (Solanum tuberosum) were selected. In the present study, stem segments from two species selected as explant source were cultured on Murashige and Skoog’s media containing various concentrations (0.2 and 0.5 mg L-1 NAA) (0.2 and 0.5 mg L-1 IAA) of auxin and (0.2 and 0.5 mg L-1 BA) (0.2 and 0.5 mg L-1 KIN) of cytokinins. Callus induction from stem segments of Jerusalem artichoke occured on most of the media tested, but the most callus formation (100%) took place on MS media containing only NAA for explants of purple potato. When the level of NAA increased in the medium, bulblet formation and shoot proliferation decreased for both species. On the other hand, lower concentrations of KIN induced shoot formation for the explants of both species. The present study reports on the effective regeneration protocol in tuberous plants tested and the outcome provides for further genetic research on Jerusalem artichoke and purple potato.