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Evalution of DNA extraction methods in order to monitor genetically modified materials in soy foodstuffs and feeds commercialised in Turkey by multiplex real-time PCR

Türkeç, Aydın and Kazan, Hande and Baykut, Aykut and Lucas, Stuart J. (2015) Evalution of DNA extraction methods in order to monitor genetically modified materials in soy foodstuffs and feeds commercialised in Turkey by multiplex real-time PCR. Journal of the Science of Food and Agriculture, 95 (2). pp. 386-392. ISSN 0022-5142 (Print) 1097-0010 (Online)

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Official URL: http://dx.doi.org/10.1002/jsfa.6731

Abstract

BACKGROUND Soybean is one of the most important biotech crops, widely used as an ingredient in both foodstuffs and feed. DNA extraction methods have been evaluated to detect the presence of genetically modified (GM) materials in soya-containing food and feed products commercialised in Turkey. RESULTS All extraction methods performed well for the majority of soya foods and feed products analysed. However, the most successful method varied between different products; the Foodproof, Genespin and the cetyltrimethylammonium bromide (CTAB) methods each produced the highest DNA yield and purity for different soya foodstuffs and feeds. Of the samples tested, 20% were positive for the presence of at least two GM elements (35S/NOS) while 11% contained an additional GM element (35S/NOS/FMV). Of the tested products, animal feeds showed a larger prevalence of GM material (50%) than the soya-containing foodstuffs (13%). CONCLUSION The best performing extraction methods proved to be the Foodproof, Genespin and CTAB methods for soya-containing food and feed products. The results obtained herein clearly demonstrate the presence of GM soybean in the Turkish market, and that the Foodproof GMO Screening Kit provides reliable screening of soy-containing food and feed products. © 2014 Society of Chemical Industry

Item Type:Article
Uncontrolled Keywords:food;feed;GM soy;DNA extraction;real-time PCR;multiplex PCR screening
Subjects:UNSPECIFIED
ID Code:26568
Deposited By:Stuart J. Lucas
Deposited On:25 Dec 2014 14:31
Last Modified:25 Dec 2014 14:31

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