title   
  

Exploring the interaction between small RNAs and R genes during Brachypodium response to Fusarium culmorum infection

Lucas, Stuart J. and Baştaş, Kubilay and Budak, Hikmet (2014) Exploring the interaction between small RNAs and R genes during Brachypodium response to Fusarium culmorum infection. Gene, 536 (2). pp. 254-264. ISSN 0378-1119 (Print) 1879-0038 (Online)

This is the latest version of this item.

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.gene.2013.12.025

Abstract

The present study aims to investigate small RNA interactions with putative disease response genes in the model grass species Brachypodium distachyon. The fungal pathogen Fusarium culmorum (Fusarium herein) and phytohormone salicylic acid treatment were used to induce the disease response in Brachypodium. Initially, 121 different putative disease response genes were identified using bioinformatic and homology based approaches. Computational prediction was used to identify 33 candidate new miRNA coding sequences, of which 9 were verified by analysis of small RNA sequence libraries. Putative Brachypodium miRNA target sites were identified in the disease response genes, and a subset of which were screened for expression and possible miRNA interactions in 5 different Brachypodium lines infected,with Fusarium. An NBS-LRR family gene, 1g34430, was polymorphic among the lines, forming two major genotypes, one of which has its miRNA target sites deleted, resulting in altered gene expression during infection. There were siRNAs putatively involved in regulation of this gene, indicating a role of small RNAs in the B. distachyon disease response.

Item Type:Article
Uncontrolled Keywords:Brachypodium distachyon; R genes; NBS-LRRs; Plant miRNAs; Plant disease
Subjects:UNSPECIFIED
ID Code:25543
Deposited By:Hikmet Budak
Deposited On:22 Dec 2014 11:54
Last Modified:22 Dec 2014 11:55

Available Versions of this Item

Repository Staff Only: item control page