title   
  

Sorting the wheat from the chaff: identifying miRNAs in genomic survey sequences of triticum aestivum chromosome 1AL

Lucas, Stuart J. and Budak, Hikmet (2012) Sorting the wheat from the chaff: identifying miRNAs in genomic survey sequences of triticum aestivum chromosome 1AL. PLoS One, 7 (7). ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0040859

Abstract

Individual chromosome-based studies of bread wheat are beginning to provide valuable structural and functional information about one of the world's most important crops. As new genome sequences become available, identifying miRNA coding sequences is arguably as important a task as annotating protein coding sequences, but one that is not as well developed. We compared conservation-based identification of conserved miRNAs in 1.5× coverage survey sequences of wheat chromosome 1AL with a predictive method based on pre-miRNA hairpin structure alone. In total, 42 sequences expected to encode conserved miRNAs were identified on chromosome 1AL, including members of several miRNA families that have not previously been reported to be expressed in T. aestivum. In addition, we demonstrate that a number of sequences previously annotated as novel wheat miRNAs are closely related to transposable elements, particularly Miniature Inverted Terminal repeat Elements (MITEs). Some of these TE-miRNAs may well have a functional role, but separating true miRNA coding sequences from TEs in genomic sequences is far from straightforward. We propose a strategy for annotation to minimize the risk of mis-identifying TE sequences as miRNAs.

Item Type:Article
Additional Information:Article Number: e40859
Subjects:UNSPECIFIED
ID Code:19384
Deposited By:Hikmet Budak
Deposited On:16 Oct 2012 12:54
Last Modified:16 Oct 2012 12:54

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