A new microfluidics system with a hand-operated, on-chip actuator for immunosensor applications
Kallempudi, Sreenivasa Saravan and Altıntaş, Zeynep and Kolkar Mohammed, Javed Hussain Niazi and Gürbüz, Yaşar (2012) A new microfluidics system with a hand-operated, on-chip actuator for immunosensor applications. Sensors and Actuators B: Chemical, 163 (1). pp. 194-201. ISSN 0925-4005
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Official URL: http://dx.doi.org/10.1016/j.snb.2012.01.034
This paper presents the realization of a portable, point-of-care and multi-target immunosensing lab-on-a-chip (LOC). The chip utilizes a novel on-chip actuating mechanism which uses a negative pressure to create thrust inside the channels to facilitate and control the fluid flow. No external instruments and/or sources were used to drive or control the liquid throughout the experimentation. LOC demonstrates a highly sensitive detection and quantification of a cancer marker, human-epidermal growth factor receptor (hEGFR) and a cardiac marker, interleukin 6 (IL-6) by impedimetric measurement approach. Without the use of any signal amplification methods (chemical), a dynamic detection limit of 3-8 ng/ml of hEGFR and 0.1-5 ng/ml of IL-6 in human serum was obtained. The extracted dissociation constants were calculated as 5.86 ng/ml (hEGFR) and 2.45 ng/ml (IL-6) by kinetic analysis.
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