title   
  

Cryopreservation of spleen and lymph nodes as a source of mononuclear cells to be used for the development of monoclonal antibody producing hybridoma cells

Sogut, İbrahim and Hatipoğlu, İbrahim and Serhatlı, Müge and Çevik, Şafak Işıl and Durali, Deniz and Başalp, Aynur (2011) Cryopreservation of spleen and lymph nodes as a source of mononuclear cells to be used for the development of monoclonal antibody producing hybridoma cells. Cryoletters, 32 (3). pp. 266-274. ISSN 0143-2044

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Abstract

In the present study, spleen and lymph nodes of mice were cryopreserved as a whole tissue and after thawing, membrane integrity of mononuclear cells was determined by trypan blue exclusion and PI staining. T and B lymphocytes, macrophages and dendritic cells have been isolated from both cryopreserved tissue and analyzed by Flow cytometry. BALB/c mice were immunized with Hepatitis e antigen (HBeAg) and spleen and lymph nodes of mice were cryopreserved for 3 to 10 months. The cells obtained from both tissue were applied to hybridoma technology to understand if the cells keep their viability and functionality. The cells were isolated and fused with F0 mouse myeloma cells and several antibody producing hybrid cells were developed. Results have shown that cryopreserved spleen and lymph nodes of mice can be efficiently used in hybridoma technology for the successful generation of monoclonal antibody producing hybrid cells.

Item Type:Article
Uncontrolled Keywords:Cryopreservation; spleen; lymph nodes; hybridoma technology; ELISA; antibody
Subjects:Q Science > QH Natural history > QH301-705.5 Biology
ID Code:16668
Deposited By:Şafak Işıl Çevik
Deposited On:12 Aug 2011 10:28
Last Modified:12 Aug 2011 10:28

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