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Frequent gene movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives

Wicker, Thomas and Mayer, Klaus F. X. and Gundlach, Heidrun and Martis, Mihaela and Steuernagel, Burkhard and Scholz, Uwe and Simkova, Hana and Kubalakova, Marie and Choulet, Frederic and Taudien, Stefan and Platzer, Matthias and Feuillet, Catherine and Fahima, Tzion and Budak, Hikmet and Dolezel, Jaroslav and Keller, Beat and Stein, Nils (2011) Frequent gene movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives. Plant Cell , 23 (5). pp. 1706-1718. ISSN 1040-4651

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Official URL: http://dx.doi.org/10.1105/tpc.111.086629

Abstract

All six arms of the group 1 chromosomes of hexaploid wheat (Triticum aestivum) were sequenced with Roche/454 to 1.3- to 2.2-fold coverage and compared with similar data sets from the homoeologous chromosome 1H of barley (Hordeum vulgare). Six to ten thousand gene sequences were sampled per chromosome. These were classified into genes that have their closest homologs in the Triticeae group 1 syntenic region in Brachypodium, rice (Oryza sativa), and/or sorghum (Sorghum bicolor) and genes that have their homologs elsewhere in these model grass genomes. Although the number of syntenic genes was similar between the homologous groups, the amount of nonsyntenic genes was found to be extremely diverse between wheat and barley and even between wheat subgenomes. Besides a small core group of genes that are nonsyntenic in other grasses but conserved among Triticeae, we found thousands of genic sequences that are specific to chromosomes of one single species or subgenome. By examining in detail 50 genes from chromosome 1H for which BAC sequences were available, we found that many represent pseudogenes that resulted from transposable element activity and double-strand break repair. Thus, Triticeae seem to accumulate nonsyntenic genes frequently. Since many of them are likely to be pseudogenes, total gene numbers in Triticeae are prone to pronounced overestimates.

Item Type:Article
Subjects:Q Science > QH Natural history > QH506 Molecular biology
ID Code:16604
Deposited By:Hikmet Budak
Deposited On:11 Jul 2011 15:47
Last Modified:11 Jul 2011 15:47

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