title   
  

Review of current methodological approaches for characterizing microRNAs in plants

Unver, Turgay and Namuth-Covert , Deena M. and Budak, Hikmet (2009) Review of current methodological approaches for characterizing microRNAs in plants. International Journal of Plant Genomics, 2009 . ISSN 1687-5370

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Official URL: http://dx.doi.org/10.1155/2009/262463

Abstract

Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenesis of miRNA in plant systems. The remainder of the paper will go into details of three different approaches used to identify and study miRNA. These include two reverse genetics approaches: computation (bioinformatics) and experimental, and one rare forward genetics approach. We also will summarize how to measure and quantify miRNAs, and how to detect their possible targets in plants. Strengths and weaknesses of each methodological approach are discussed.

Item Type:Article
Subjects:UNSPECIFIED
ID Code:13599
Deposited By:Hikmet Budak
Deposited On:22 Dec 2009 09:33
Last Modified:22 Dec 2009 09:33

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