Differential expression of wheat transcriptomes in response to varying cadmium concentrations
Cebeci, Özge and Köktürk, B. and Ergen, Neslihan and Öztürk, Levent and Çakmak, İsmail and Budak, Hikmet (2008) Differential expression of wheat transcriptomes in response to varying cadmium concentrations. Biologia Plantarum, 52 (4). pp. 703-708. ISSN 0006-3134 (Print) 1573-8264 (Online)
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Official URL: http://dx.doi.org/10.1007/s10535-008-0135-z
This study aims to understand the changes in the transcriptome of durum wheat (Tricitum durum cv. Balcali-85) upon exposure to varying Cd concentrations using mRNA differential display (mRNA DD) technique. Sequence analyses of the two heavily induced genes upon exposure to Cd showed high homology to NADH dehydrogenase subunit 1 (EC907725) and PsaC gene encoding a photosystem 1 (PS 1) 9 kDa subunit protein (EC907731). Additionally, three differentially expressed genes (EC907726, EC907729 and EC907730) were identified. Their sequence analyses revealed no significant homologies to known genes. The expressions of NADH dehydrogenase subunit 1 and PsaC genes were confirmed by Northern blot analysis and quantified by real time PCR. This is the first report for the induction of NADH dehydrogenase subunit 1 gene during Cd stress in wheat.
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