title   
  

Differential expression of wheat transcriptomes in response to varying cadmium concentrations

Cebeci, Özge and Köktürk, B. and Ergen, Neslihan and Öztürk, Levent and Çakmak, İsmail and Budak, Hikmet (2008) Differential expression of wheat transcriptomes in response to varying cadmium concentrations. Biologia Plantarum, 52 (4). pp. 703-708. ISSN 0006-3134 (Print) 1573-8264 (Online)

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Official URL: http://dx.doi.org/10.1007/s10535-008-0135-z

Abstract

This study aims to understand the changes in the transcriptome of durum wheat (Tricitum durum cv. Balcali-85) upon exposure to varying Cd concentrations using mRNA differential display (mRNA DD) technique. Sequence analyses of the two heavily induced genes upon exposure to Cd showed high homology to NADH dehydrogenase subunit 1 (EC907725) and PsaC gene encoding a photosystem 1 (PS 1) 9 kDa subunit protein (EC907731). Additionally, three differentially expressed genes (EC907726, EC907729 and EC907730) were identified. Their sequence analyses revealed no significant homologies to known genes. The expressions of NADH dehydrogenase subunit 1 and PsaC genes were confirmed by Northern blot analysis and quantified by real time PCR. This is the first report for the induction of NADH dehydrogenase subunit 1 gene during Cd stress in wheat.

Item Type:Article
Additional Information:cadmium-responsive genes - functional genomics - mRNA differential display - Triticum durum
Subjects:UNSPECIFIED
ID Code:10453
Deposited By:Hikmet Budak
Deposited On:11 Nov 2008 13:48
Last Modified:25 May 2011 14:08

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